Stéphane Dupas

Scientist
Stéphane Fraichard

General information

ITA

TPI – Taste and Post-ingestive Integration

 Stephane.dupas@u-bourgogne.fr
 No Hal

Job

CNRS, Assistant Researcher

  • 2014 : Bsc of Applied Biology, Kingston University, United-Kingdom
  • 2013 : Master's degree in cell biology, Poitiers, France

Activities

Research theme
Stéphane has been a project engineer at the CNRS for over 25 years. He first worked for just over a year at the Institut Georges Hayem (Hôpital Saint-Louis, Paris) on the nuclear receptor RARalpha. He then spent 8 years at the Ecole Normale Supérieure in Paris in a laboratory studying the Engrailed protein, a protein involved in neuronal growth in mice. He came to Dijon in 2006 to study various genes closely or remotely involved in the perception of odoursor taste. Since the start of his career, he has had the opportunity to learn and use a wide range of molecular biology techniques: PCR, Q-PCR, Western blot, Northern blot, Southern blot, imunohistochemistry, cloning and sub-cloning, production of transgenic Drosophila lines using Crispr-Cas9 tools (deletion of genes and clusters (several genes at once), addition of a tag to proteins), production and purification of proteins, etc., as well as techniques for the analysis and analysis of proteins. As well as behavioural techniques on Drosophila (Ethovision, etc.). Theoretical and practical knowledge that he is happy to teach to students coming to the laboratory for work placements. He has set up a working group on the environmental impact of the CSGA and spends time raising awareness among his colleagues and finding solutions to reduce the CSGA's carbon footprint.
Scientific approach
Behavior, molecular biology, genetic
Supervision
Stéphane supervised numerous students.
Key words
Taste, post-ingestion, neurosciences, Drosophila, behavior, metabolism, memory

Important publications

  • Alves G, Sallé J, Chaudy S, Dupas S, Manière G (2014). High-NaCl perception in Drosophila melanogaster. J Neurosci 2014 Aug 13;34(33):10884-91
  • Bousquet F, Nojima T, Houot B, Chauvel I, Chaudy S, Dupas S, Yamamoto D, Ferveur JF (2012). Expression of a desaturase gene, desat1, in neural and nonneural tissues separately affects perception and emission of sex pheromones in Drosophila. Proc Natl Acad Sci U S A. Jan 3;109(1):249-54 Grange J,
  • Belly A, Dupas S, Trembleau A, Sadoul R, Goldberg Y (2009). Specific interaction between Sam68 and neuronal mRNAs: implication for the activity-dependent biosynthesis of elongation factor eEF1A. J Neurosci Res 2009 Jan;87(1):12-25.
  • Parrado A, Despouy G, Kraïba R, Le Pogam C, Dupas S, Choquette M, Robledo M, LargheroJ, Bui H, Le Gall I, Rochette-Egly C, Chomienne C, Padua RA (2001). Retinoic acid receptor alpha1 variants, RARalpha1DeltaB and RARalpha1DeltaBC, define a new class of nuclear receptor isoforms. Nucleic Acids Res. Dec 15;29(24):4901-8.
  • Joliot A, Maizel A, Rosenberg D, Trembleau A, Dupas S, Volovitch M, Prochiantz A (1988). Identification of a signal sequence necessary for the unconventional secretion of Engrailed homeoprotein. Curr Biol. Jul 16;8(15):856-63.

TPI – Taste and Post-ingestive Integration